The project aims to investigate the transcriptional differences between intestinal organoids cultured under normoxic (21% O2) and hypoxic (1-5% O2) conditions, with a particular focus on the involvement of the transcription factor HIF-2α. Using available RNA sequencing data from previously published studies on intestinal organoids, the project will compare the gene expression profiles with newly generated RNA sequencing data from our lab’s organoid cultures at hypoxia. The project aims to elucidate the impact of oxygen levels on the HIF-2α pathway and its target genes in the context of intestinal stemness and tissue renewal.
The project will identify key genes, pathways, and biological processes that are differentially regulated in response to hypoxia. It will extend to the identification and characterization of HIF-2α target genes, with a specific emphasis on their role in modulating the fundamental processes associated with intestinal stemness and tissue renewal.
Moreover, utilizing advanced bioinformatics tools, the project will employ Gene Set Enrichment Analysis (GSEA) to discern the enrichment patterns of HIF-2α target upregulated genes within the obtained datasets. This approach will provide a comprehensive understanding of the potential implications of HIF-2α in intestinal homeostasis.
Contact: Paula Martos Salvo, paula.martos_salvo@med.lu.se