We are looking for highly motivated students to be part of a project to optimize a cost-effective pipeline to produce recombinant humanized antibodies in mammalian cells as diagnosis tools for immune inflammatory responses and complement pathway related.
Brief project description
This master’s project is part of a larger PhD project for optimizing the affinity of recombinant antibodies and designing new antibody formats, such as nanobodies, ScFv, and full antibodies, with a higher half-life and better yield. This includes the discovery of new antibodies from immunized mouse and further high-throughput screening by the cloning antibody tool. The project involves the use of sequencing techniques from antibody-expressing cells, Golden-Gate-based molecular cloning, plasmid design, PCR amplifications, cell culture for expression in CHO cell lines and purification techniques. Once this pipeline is well established and optimized, the antibodies will be characterized and tested in ELISAs and functional assays for diagnostic, experimental and potentially therapeutic potential. We will investigate the complement system activation and inhibition related to these diseases, producing recombinant antibodies against complement proteins such as C1s, MASP-2, Factor D or C5aR1. The project also allows the student to be part of a publication to disseminate the results derived from this research.
Who are we looking for?
We seek an excellent student with a good understanding of biochemistry and molecular biology. Previous experience in a laboratory working with microbiology, cell culture and methods such as PCRs, ELISAs and Western blots will be valued, but it is not a requirement. We prioritize proactive students with teamwork skills and enthusiasm to learn with great interest and motivation in biomedical research.
Who are we?
We are a research group of around 15 people led by Professor Peter Garred and part of the Department of Clinical Immunology at Rigshospitalet and University of Copenhagen. Our focus is innate immunity, with a particular interest in the complement system’s role in health and disease. The group atmosphere is international, informal and friendly, and we are prepared to give you substantial supervision and support regarding your project.
Contact:
Group leader Professor Peter Garred
Please include your CV and transcripts in the email.