Biology Education

Department of Biology | Lund University

Grid View

Plant-Fungal symbiosis, biostimulation and antibiotic resistance

Project suggestion

Supervisor: Allan Rasmusson, Molecular Bioscience, Dept. Biology, LU

Many fungal species of the genus Trichoderma live in symbiosis with plant roots. The fungus produces substances that stimulate plant growth and immune system but also directly attacking other microorganisms, including pathogens. Therefore, some strains of Trichoderma have been used as biocontrol and biostimulants in agriculture, decreasing the need for agrochemical use. The effect of Trichoderma on plants is strongly dependent on the plant genome, which is involved in a mainly unknown intricate interaction with the fungus, likely involving exchange of several signalling biomolecules.  Plant genes that are essential for biostimulation and biocontrol by fungi can be used in breeding, to make plants that can better benefit from biocontrol and biostimulation. However, we presently do not know what genes these are.

One possible class of genes that are essential for positive symbiosis with fungi are plant genes encoding proteins that are needed for the plants to avoid being damaged by the Trichoderma. This fungus attacks other microorganisms by secreting enzymes and peptide antibiotics, including so called peptaibols, where alamethicin is the standard model example. This peptide also lyses plant cells, but cellulase secreted from the Trichoderma induces resistance to the alamethicin by modifying the composition of the plant plasma membrane. This process we have named CIRA, and it is likely important for plant symbiosis with Trichoderma, though direct evidence are lacking. We have isolated CIRA-deficient mutants for 20+ Arabidopsis genes, indicating that a so far unknown response chain is active. The different mutants belong to the categories Gene expression, Cell wall modifying, Signalling, Membrane lipid modifying and “Unknown”. Identification and analysis of novel CIRA genes by mutant screening is a way to increase our knowledge of this process and possibly build a scheme for the involvement of the different genes.

Differently long projects (30-60 credits) can be designed within the area of:

  • Analysis of particular known Arabidopsis mutants, their (lack of) biostimulation by Trichoderma, and the potential involvement of calcium signalling. Methodologically, this will involve at least sterile plant growth, fluorescent microscopy and measurements using intracellular fluorescent reporters.
  • Identification and analysis of novel CIRA genes by mutant screening. Arabidopsis CIRA mutants are identified by a fluorescent phenotypic change and verified by designing PCR assays and analysing a second mutant allele for the same gene. The genes found are analysed in a first line by bioinformatic database mining (e.g. gene expression patterns, protein localisation, post-translational modification, etc). In a longer project additional analyses can be done. Acquired data will be used in order to assemble a preliminary signalling path.

For more information and discussions please contact allan.rasmusson@biol.lu.se

Most suitable background knowledge is molecular cell biology/molecular physiology with a focus on plants, yet with some understanding of plant interactions with other organisms.

Length of project: Flexible, a project of 30-60 credits can be designed.

Start date: To be discussed

 

January 28, 2026

This entry was posted in

Biology Molecular Biology

Comments

0 Comments Leave a comment

Optimizing AAV Genomic Architecture for Improved Functionality

Project information:
Recombinant adeno-associated virus (rAAV) vectors are among the most promising platforms for gene therapy, but their performance depends strongly on how the AAV genome is designed. Small changes in genomic architecture can influence expression levels, stability, packaging efficiency, and overall functionality—factors that directly impact therapeutic potential.
In this project, you will investigate how specific genome modifications affect rAAV vector performance. By designing and building new constructs and evaluating them in cell-based assays, you will contribute to the development of next-generation AAV tools with improved functionality for future gene therapy applications.

Objectives:
This master’s level project (60 ECTS) aims to design, generate, and evaluate modified AAV genome constructs to understand how genome architecture influences vector performance. You will assess expression and stability using molecular and functional readouts and compare results across designs to identify improved configurations.

Your role:

  • Design AAV genome constructs and plan cloning strategies
  • Perform molecular cloning and construct assembly (plasmid design and building)
  • Validate constructs using quality control methods (e.g., sequencing, restriction analysis)
  • Run mammalian cell culture and transfection experiments
  • Perform cell-based expression and functionality assays to evaluate construct performance
  • Analyze and interpret experimental data, summarize results, and propose next-step designs

This project offers hands-on experience with real-world gene therapy development challenges, combining design thinking with experimental testing. You will work in a biotech environment where your results can directly guide improved vector development.

Required knowledge:
Basic laboratory skills and motivation to work independently are essential. Experience in molecular cloning is beneficial, and prior exposure to mammalian cell culture is a plus. A strong interest in gene therapy, synthetic biology, or biotech innovation will help you thrive in the project.

Who should apply:

  • Students interested in gene therapy, vector engineering, or synthetic biology
  • Curious minds who enjoy troubleshooting and optimizing biological systems
  • Students who like combining lab work with data exploration and interpretation
  • Prior cloning experience is helpful but not required with the right motivation

Project duration: 60 ECTS

Location:
rAAVen Therapeutics, Medicon Village, Lund

Start date:
Flexible / by agreement

What you’ll gain:

  • Practical experience in cutting-edge biotech and gene therapy development
  • Deep understanding of vector design principles and experimental optimization
  • Insight into how startups operate in an innovation-driven environment
  • Opportunities to attend talks and conferences
  • Optional involvement in additional lab work depending on interest and timing
  • The chance to contribute to technology with real impact in gene therapy innovation

Contact

marcus.davidsson@raaven.se

www.raaven.se

 

January 26, 2026

This entry was posted in

Molecular Biology

Comments

0 Comments Leave a comment

Bumblebee foraging within a robotically-controlled virtual reality: How do miniaturebrains memorize complex environments?

Bees are extraordinary navigators — using only a few learning flights, bees are able to build internal memories of their visual surroundings that allow them to efficiently locate previously-discovered food sources as well as their hive. In this exciting project, we aim to gain a deeper understanding of how bumblebees acquire and process navigational memories by letting bees forage in a virtual, computer-generated environment. Although the bees are tethered in place, they are able to move through a computer-generated environment by walking on an air-suspended ball. We will subsequently provide robotically-controlled food rewards to encourage visual learning. We will leverage the unique experimental flexibility given to us by the virtual reality technology to probe features of the bees visual memories, e.g. by virtually displacing bees off of their previously-learned trajectories. This interdisciplinary project combines hardware prototyping and behavioral data collection. Do you like solving technical problems, coding or robotics, and want to apply these skills to exciting fundamental questions in neuroscience and biology? Then this project is especially suited to you! Prior experience with either Python, C, and/or technical prototyping is advantageous, but we encourage anyone interested in gaining these skills to apply. For an informal chat about the project possibilities, please send us a note!

This project can be adapted to 30, 45 or 60 credits, although 45-60 credits is ideal. The
project can be adjusted to either an MA or BA thesis. We are open to hearing your
preferences.

Start date
The start date for this project is flexible, ideally between February 15 and April 1.

Contact info:
Abel Corver, abel.corver@biol.lu.se
LU research profile: https://portal.research.lu.se/en/persons/abel-corver/
abelcorver.com

January 25, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Eavesdropping on underground conversations: Investigating secondary metabolites secreted during interspecific interactions of soil fungi

Project information: Soil fungi are key drivers of biogeochemical cycles through their roles in organic matter decomposition and plant nutrition via mycorrhizal symbioses. Their impact is based on both their diverse lifestyles and their interactions and competition for space and resources. These interactions can strongly affect decomposition rates and fluxes of carbon in soils, as well as plant productivity. However, the molecular and biochemical mechanisms underlying soil fungal interactions, and how they are shaped by environmental conditions, remain poorly understood. Since climate change-induced shifts in vegetation affect the composition of fungal communities in the soil, understanding these mechanisms is essential for predicting future decomposition dynamics and soil carbon release.

Objectives: This master’s level project (30–45 ECTS) will explore interactions between fungal species, focusing on how secreted bioactive compounds shape the outcomes of fungal confrontations. The aim is to examine catalogs of fungal compounds secreted during confrontation for biologically active metabolites, isolate them, and analyze their effects at the mycelium and single hyphae levels.

Your role:

  • Co-culture different fungal species and extract their secreted metabolites.
  • Use high-performance liquid chromatography (HPLC) to separate fungal extracts into fractions and ultimately (few) single compounds.
  • Perform bioactivity tests on fungal agar plate cultures as well as in special microfluidic chips which allow microscopic observations of single hyphae.

This project offers the opportunity to apply molecular and biochemical methods to key questions in soil microbiology and potentially contribute to discovery of novel biochemical compounds. You will apply basic microbiological techniques as well as more specialized analytical methods and explore new approaches such as bioactivity testing in microfluidic chips. Your interests and ideas are welcome and can contribute to the direction of the project.

Required knowledge: Basic laboratory skills, preferably with experience working in sterile conditions. Strong interest in soil microbiology and fungi and biochemical lab techniques.

Start date: Any time from mid-March 2026 onwards

Interested? Contact us: Valentina Wolf (PhD candidate): valentina.wolf@biol.lu.se, Valentina Wolf – Lund University; Main supervisor: Milda Pucetaite, Milda Pucetaite – Lund University 

January 23, 2026

This entry was posted in

Biology Molecular Biology

Comments

0 Comments Leave a comment

Same flower, different smell? Exploring scent polymorphism and community context in deceptive orchids

Many flowering plants rely on visual and chemical signals to attract pollinators. Food-deceptive orchids attract their pollinators without offering nectar rewards, instead exploiting pollinator sensory expectations. Dactylorhiza sambucina is a food-deceptive orchid with two flower colour morphs that coexist within populations, a polymorphism thought to be maintained by negative frequency-dependent selection. While colour variation in this species is well studied, it remains unknown whether the morphs also differ in floral scent, a key but often overlooked component of pollinator attraction.

This project aims to investigate whether variation in floral scent parallels colour polymorphism and whether the co-flowering community context, including other food-deceptive orchids as well as rewarding and non-rewarding plant species, contributes to maintaining colour and/or scent variation within populations through pollinator-mediated interactions.

Appreciated knowledge: background on pollination biology or plant ecology is desirable but not required.

  • Motivation to conduct fieldwork (approx. 2.5 months on the beautiful Öland)
  • Willingness to learn floral scent chemical analysis (GC–MS, chromatogram integration)
  • Willingness to learn multivariate data analysis in R

Length of the project: MSc, 45-60 credits.

Start date: end of April/beginning May 2026.

Contact info: Yedra García (yedra.garcia_garcia@biol.lu.se), Øystein Opedal (oystein.opedal@biol.lu.se), Magne Friberg (magne.friberg@biol.lu.se)

https://portal.research.lu.se/en/persons/yedra-garcia-garcia/

January 22, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Understanding the Impact of Urbanization on the Production of Avian Ejaculate

The average reproductive performance of birds has been shown to be lower in polluted areas (Eeva & Lehikoinen, 1995; Peach et al, 2008, Barton et al, 2023). Hatching success is one of the most important determinants of fitness, and the number of eggs that fail to hatch can have a serious impact on population dynamics (Ferreira et al, 2005; White et al, 2015). Hatching failure (excluding predation and abandonment) can have different proximate causes: for example, it may result from poor sperm quality, bad female condition, and/or environmental factors.

Many vertebrates, such as birds, undergo annual cycles of gonad development in which the gonads must develop before breeding can commence (Davies and Deviche, 2014). It was shown that urban birds have earlier gonadal development compared with their rural conspecifics (Davies et al, 2015). It was shown in human that higher exposure to air-pollutant can cause damage to testes, characterized by histological alterations, oxidative damage and apopotosis. However, we know that urbanization has an impact on the timing and pace of gonadal development, we do not know how it can influence the process of sperm production and the quality of sperm. Therefore, the proposed project will address how urbanization and traffic related pollutants can influence the oxidative status (SOD activity, MDA level and T-AOC) and apoptosis level of the testes and the ejaculate in wild birds, and how it influences the quality of sperm (swimming velocity, progressive motility, morphology and DNA damage).

Project will be carried out on Blue and Great tits (Cyanistes caeruleus and Parus major), which makes possible that the students are working together closely during the course of their master degree, while still doing their independent research.

 

Master project 1 & 2 (MP12)

Objective

To determine whether urbanisation influences sperm quality via oxidative damage in the testes

Your role

You investigate whether the oxidative status and the apoptosis level in testes influences the quality of the sperm.

 

Master project 3 & 4 (MP34)

Objective

To determine whether urbanisation influences sperm quality via oxidative damage in the ejaculate.

Your role

You investigate whether the oxidative status of the ejaculate influences the quality of the sperm.

 

Fieldwork & data collection

Participating in long-term data collection by monitoring nest boxes in 5 different parks of Malmö, and in Skrylle Nature Reserve (MP1234).

– Catching, handling and ringing birds using various methods (MP1234).

– Collect blood and ejaculate samples (MP1234) and organs including the testes (MP12).

– Measuring sperm swimming velocity and progressive motility (MP1234).

 

Labwork

– Measuring SOD, MDA, T-AOC and apoptosis levels from the testes (MP12).

– Measuring SOD, MDA, T-AOC and apoptosis levels from the ejaculate (MP34).

– Measuring sperm morphology and DNA damage (MP34).

 

Length of the projects

10 months theses for 60 credits are prioritized, but shorter theses may be organized as well, depending on the project.

 

Start date

The fieldwork starts at the beginning of April 2026.

 

Requirements

Participating students must take the Animal Testing Ethics course before the start of the project.

 

Contact info

Zsófia Tóth – zsofia.toth@biol.lu.se

https://portal.research.lu.se/en/persons/zsófia-tóth/

January 22, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Experimental evolution of multicellularity in green algae

Background

Across the tree of life, multicellularity has repeatedly evolved within some clades, such as the green algae, but not within other clades. Why has multicellularity evolved so many times in some lineages, but not others? In this project, we want to test the idea that multicellularity readily evolves from unicellular organisms that prolong their replicative (multicellular) life-stages under specific environmental conditions (“facultative multicellularity”). This project could yield new insights in how plasticity in the unicellular ancestors of multicellular green algae has preceded the evolution of obligate multicellularity.

Methods

  • Experimental evolution of multicellularity using unicellular green algal lab cultures
  • Machine learning classification of cell images
  • Fluorescence measurements of cell growth
  • Flow cytometry cell sorting to select for multicellularity

Learning opportunities

  • Experimentation with green algal cultures
  • Using state-of-the-art equipment for data collection
  • Classify images with existing machine learning models
  • Bayesian statistics
  • Interplay of phenotypic plasticity and evolvability

Candidate profile

  • Interest in conceptually motivated questions in evolutionary biology
  • Basic knowledge of R and statistics
  • Ability to work independently with support from the supervisors
  • At least 45 ECTS project, but preferably 60 ECTS (start date flexible)

Contact details: Jan Kreider, jan.kreider@biol.lu.se; Charlie Cornwallis, charlie.cornwallis@biol.lu.se

January 21, 2026

This entry was posted in

Biology Molecular Biology

Comments

0 Comments Leave a comment

Nocturnal bird migration under the sun

Bird migration is to a large degree a nocturnal affair, as many birds start their migratory flights soon after sunset and fly until early morning. It is however unclear how this pattern is affected by the large differences in night length that birds experience at different latitudes. When migrating at high latitudes the midnight sun means that there is no “night” at all for parts on the year, so what does this mean for the nocturnal schedule?

Using weather radar data we can identify the start and end of bird migratory movements at 14 different sites in Sweden, ranging from Kiruna to Ängelholm. Comparing the length of migratory activity at different night lengths can give us clues to what cues birds use to initiate migration, and why so many birds migrate during night at all. I am looking for a motivated student analyze weather radar data of migratory activity in relation to night length at different sites and times.

Required knowledge: Comfortable with, or willing to learn, to analyse data in R.

Length of the project:  MSc or BSc, flexible depending on depth of analysis, number of sites included.

Start date: Flexible

 

Contact info: Cecilia Nilsson, Cecilia.nilsson@biol.lu.se

Link to research profile:

http://cnilsson.science

https://portal.research.lu.se/sv/persons/cecilia-nilsson

January 20, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Animal behavior during a solar eclipse

Light is a powerful cue that guides and controls much of life on earth. Sunlight also follows a predictable and steady schedule, at least most of the time. So, what happens when it suddenly doesn’t? In 2017 we used large-scale weather radar data to investigate how animals in the air reacted to the 2017 US total solar eclipse. Across 143 radar sites we measured drops in animal activity in the air as the eclipse passed, probably mainly due to flying birds landing. At sites in the path of totality we saw a slightly different reaction, with some bursts of activity, possibly related to insect activity.

In 2024 another total eclipse occurred across the US. This eclipse had a different path, meaning different radar sites experienced totality, and it also occurred in a different season, spring rather than autumn. We now have the data for the 2024 eclipse and are looking for a motivated student to compare animal activity in the air between the two eclipses.

See more: https://doi.org/10.1098/rsbl.2018.0485

Required knowledge: Comfortable with, or willing to learn, to analyse data in R.

Length of the project:  MSc, flexible depending on depth of analysis 

Start date: Flexible

 

Contact info: Cecilia Nilsson, Cecilia.nilsson@biol.lu.se

More information:

http://cnilsson.science

https://portal.research.lu.se/sv/persons/cecilia-nilsson

January 20, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Do you like plants, pollinators and berries? Are you interested in doing fieldwork in the Arctic? Global changes and the pollination of berry-producing shrubs in the Arctic

Global changes, including climate warming and habitat change, are posing unprecedented and accelerating threats to biodiversity and functioning of high-altitude and -latitude ecosystems. Nonetheless, little is known about influence of global changes affect biotic interactions in these regions. In this project, we investigate the effects of climate warming on pollination along an altitudinal gradient using a space-for-time substitution design. We focus on two charismatic keystone plants species, bilberry (Vaccinium myrtillus) and lingonberry (V. vitis-idea), which are used for berry picking and require buzz pollination for maximising their reproductive outputs. Bumblebees, abundant at high-altitude and –latitude can perform buzz pollination and are the main pollinators of these plants. Fieldwork is done on Mount Nuolja, a mountain from Abisko National Park, Sweden. Alongside monitoring of the bumblebee community pollinating these two plants, part of the work focuses on the variation of the the plants’ reproductive success.

Your role: For the spring/summer 2026, I you will investigate through fieldwork and field experiments:

  • the changes in pollinator communities with habitat/altitude. This will be assessed through transect walks in different habitats (birch forest and open low-altitude for bilberry; low-altitude and high-altitude open habitat for lingonberry).
  • the variation in insect-pollination dependency, pollen limitation and reproductive success with climate. This will be assessed by a pollination experiment at different altitude including: (i) open pollination – flowers are available to the pollinators, (ii) bagged – flowers can only be wind- or self-pollinated, (iii) pollen supplementation by hand-pollination. This experiment will allow calculating the pollen limitation. The berries of treatment (i) will be used to assess the effects of climate. We will use berries from treatments (ii) and (iii) to assess the effects of insect pollination (within site comparison) and the combination of insect pollination and climate (between altitude comparison). This will be achieved by harvesting and drying berries for measuring their dry weight. We will count seed set (i.e., number of seeds per fruit) for a subset of collected berries.

 

Required knowledge:

  • Experience of fieldwork, preferentially in boreal/Arctic conditions
  • Familiar with community modelling
  • Motivation/interest to spend 3 months in Abisko.

 

Appreciated knowledge:

  • Knowledge of pollination ecology
  • Naturalist knowledge of pollinators/insects
  • Knowledge/interest of the effects of global changes – particularly of climate change – on the Arctic and/or pollinators/pollination

 

N.B: Fieldwork in the Arctic is physically demanding. Living in Abisko field station involves sharing accommodation (including bedroom) with other fieldworkers.

 

Length of project: project of 45 to 60 credits preferred – given the 3 months of fieldwork.

 

Starting date: April/May 2026

 

Contact: Océane Bartholomée at oceane.bartholomee@mgeo.lu.se.

https://www.cec.lu.se/oceane-bartholomee

 

Please include a brief statement (a few sentences) about your background, interests, and motivation behind why you would like to be part of the project.

 

Thank you!

January 20, 2026

This entry was posted in

Biology

Comments

0 Comments Leave a comment

Newer Posts Older Posts